The building blocks of the seeley family

DNA Project

Connecting the Seeley family in a powerful way—confirming relationships, discovering new relatives, and tracing our roots back to the original ancestor turns history into something personal, creating bonds that span generations.

Revealing Our Genetic Connections

Introduction to the DNA Project

DNA plays a critical role in genealogy because it provides a biological record of ancestry that complements traditional paper-based research. Unlike historical documents, which can be incomplete or inaccurate, DNA offers scientific evidence of family relationships, ethnic origins, and ancestral migration patterns. By analyzing genetic markers, individuals can confirm lineage, identify unknown relatives, and break through genealogical ‘brick walls,’ making DNA an indispensable tool for building accurate and comprehensive family histories.

In 2002, Fred Hart, a professional genealogist hired by SGS, had just finished some research on immigrant Obadiah Seeley (SGS #4) and suggested that we might try using DNA testing to gain further information about Obadiah's ancestry in England.  The Board agreed and Wesley Waring, SGS member, took on the project.  Eight SGS members did the first round of testing and results were received in 2003.

Immigrants Robert Seeley and Obadiah Seeley Are Not Related

The DNA Project has discovered that Robert and Obadiah were not father/son nor brothers.  According to the DNA results, Robert and Obadiah were not even closely related.  DNA markers between the two are significantly different based on the testing of descendants with clear evidence back to these original immigrants.

Project Goals

This project has two main goals:

  1. To clearly establish the 37-marker Y-chromosome DNA profiles for the various Seeley immigrants (all spellings), including Robert/Nathaniel, Obadiah, and the others, for example the Isle of Shoals Seeley/Cilley family.
  2. To help researchers to either establish their connection to one of the immigrants or to confirm their conventional genealogical research using DNA data.

Many researchers do not have their Seeley lineages confirmed back to the immigrant. For those people, DNA can clearly show if they are descended from either Nathaniel or Obadiah. All but one of the Seeleys tested to date have been found to be descendants of Obadiah or Nathaniel.

When we have obtained enough DNA data from individuals with known lineages, we may be able to use the DNA data to identify the first several generations down from an immigrant using a person’s DNA profile. Mutations (random changes in the DNA profile between generations), happen rarely, but often enough to be found during the number of generations between the immigrants and the living generations. These changes are passed down from father to son. When enough data is collected, it should be possible to say something like “you are most likely on the Obadiah-Jonas line because you have a score of 16 at DYS456” (just a hypothetical example, not clearly shown yet).

Tracing DNA Back to:

Nathaniel and Obadiah

The DNA Project initially started with the assumption that Nathaniel (SGS #2) and Obadiah (SGS #4) were both the sons of immigrant ancestor Robert Seeley (SGS #1) and set out to prove that connection. Therefore, the project focused it's DNA research on known descendants of Nathaniel and Obadiah instead of Robert. It was resoned that focusing on Robert descendants would not have provided the specificity being sought. The project results, therefore, show lineage to Nathaniel and Obadiah.

The project proved, however, that Nathaniel and Obadiah were not even related. Therefore, DNA results leading to Nathaniel also lead to Robert. And we now know that Obadiah too was an immigrant with DNA results leading to him leading to ancestors not yet firmly established - potentially to William Seeley in England.

DNA Test Results

Testing started in 2003 with eight SGS Members and has expanded ever since.  

Seeley DNA Lineage

View the results of the original Y-DNA testing.
(Relative Genetics)
See Results

Project Results

Seeley DNA testing results appear in two different places: In a table on our website and on a FamilyTreeDNA website as a specific project. More information on reading these tables is forthcoming, but suffice it to say at the moment that both tables contain the results of the same DNA testing. The FamilyTreeDNA results are far more detailed, however, and is the better resource. Though both contain accurate data.

FamilyTree Seeley DNA

The project was expanded to include different DNA tests and to include our female descendants.
See Results

Join the DNA project

Discover Your Roots. Connect Your Story.

Have you ever wondered how your DNA ties you to the Seeley family story—or to an original immigrant ancestor? Our DNA Project is your chance to find out.

Confirm your family connections and fill in missing branches of your tree.

Discover shared ancestry with other members and deepen your understanding of your heritage.

Help build a stronger Seeley family network for generations to come.

Every test adds a piece to the puzzle, helping all of us better understand where we come from. Your contribution matters—your DNA could hold the key to unlocking new family connections and breaking down brick walls!

Project Scope

The Seeley Genealogical Society's original project was to prove the lineage of Robert Seeley (SGS #1) and Obadiah Seeley (SGS #4) using the 37-marker Y-DNA test. We now have the 37-marker “Nathaniel Profile” (son of Robert) and “Obadiah Profile” clearly established. We still need to test additional Seeley-surnamed males to help establish the DNA profile for lines descended from immigrants other than Nathaniel or Obadiah.

We've now expanded the project to include all DNA tests: Y-DNA, autosomal DNA, and mitochondrial DNA (mtDNA).  Our objectives of including the autosomal and mitochondrial tests is to enhance our previous research and explore our maternal lines.  We hope this will eliminate some of the brick walls we have encountered.

If you fall into any of these categories, please consider participating in the DNA project:

  • Have a well documented lineage back to Nathaniel or Obadiah
  • Have a well documented lineage back to any other Seeley immigrant
  • Want to confirm your research using DNA
  • Don’t know the identity of your Seeley immigrant ancestor

Getting Your DNA test

We recommend using FamilyTreeDNA as your testing laboratory.

Contact the SGS DNA Project Manager, Kara Seelye Donaldson, before ordering any tests.

Y-DNA Testing (paternal line)

To be tested, you need to either be a Seeley-surnamed male (any spelling), or find a living Seeley-surnamed male on your line to test.  You whould order at least the 37 marker Y-DNA test to be able to compare your results with the DNA profiles we have on file. The Y-Chromosome is passed only from father to son, and only males with a Seeley surname should apply for the test. As of August 2025, the 37 marker Y-DNA cost $119.00 at FamilyTreeDNA and there is no SGS surcharge or cost to compare your results.

mtDNA Testing (maternal line)

With the advances in DNA testing, FamilyTreeDNA now offers Mitochondrial (mtDNA) tests on their website. Although, males do receive mitochondrial DNA from their mothers, they do not pass it on to their children, but mothers do pass it on to their daughters, which might help your research through the females in the family. SGS is now including these results along with the Y-DNA.  As of August 2025, the mtDNA test cost $159.00.

Autosomal DNA Testing

Autosomal DNA testing examines non-sex chromosomes inherited from both parents, providing information about ancestry from all branches of a person's family tree. This type of test is useful for identifying relatives across recent generations, building family trees, and estimating ethnic background. Both sexes can participate in this testing.

Testing Expenses

At the present, participants will need to pay for the cost of their own testing. In the near future, some funding may be available from the Society to help with the cost of testing.

Privacy

The identity of the test subjects will be kept strictly confidential. Participants will only be identified by the first few generations of their Seeley lineage. Participants who are close matches with other participants can be put in contact with each other to share genealogical information if both parties agree to it.

Ready to make history?

Genealogy is an ongoing journey, full of unexpected discoveries. The information here reflects our best knowledge at the moment, but it may have gaps or the occasional error.